NMR & Mass Spectrometry Facility

• UltraShieldTM Plus 9.4T magnet
• 5 mm BBFOPLUS probe
• Control temperature unit (from -50°C to 50°C

• Structure verification and elucidation
• Molecular mobility: determination of conformation in solution
• Determination of enantiomeric excess
• Kinetic and temperature studies of reaction mixtures

This facility could be used to probe materials and biological processes at the molecular and nanoscale to obtain information on the 3D structures and dynamics of molecules. This instrument is used by chemists, materials scientists, biologists and clinicians. NMR spectrometry is used to study everything from DNA to disease–causing proteins. Information obtained from NMR studies aids in the development of new drugs. NMR Spectroscopy is used to study molecular conformation in solution, quantitative analysis of mixtures, determining the content and purity of a sample.

Liquid Chromatography

• Binary and quaternary UPLC systems
• Nano-LC chip cube systems for low-flow proteomics
• Equipped with temperature controlled auto samplers
• Diode array detector

Agilent 6550 iFunnel QTOF

• Jet stream Dual ESI and APCI sources
• Extended Mass range: m/z 100-10,000
• Femtogram-level sensitivity
• Unbiased Metabolomics Platform

Agilent 6490 iFunnel MS/MS TripleQuad

• Jet stream Dual ESI and APCI sources
• Mass range: 5-1400 amu
• 500 MRMs per second, >40,000 MRMs possible in a method
• Targeted Metabolomics Platform

• Qualitative and Quantitative analyses in pharmaceutical, clinical, food safety, and environmental applications
• Proteomics Analysis

Molecules can be identified based on their accurate mass. These equipment can be used to assess and quantify metabolites and proteins in patient samples, animal models, and cell culture (including plants & fungi). These facilities can also be used to identify and quantify transient changes which influence protein function, so as to elucidate the molecular mechanisms/ pathways at work.

• Mass range: m/z 1.5 to 1000
• Ionization mode: EI
• Pump: Turbo molecular pump (58 L/sec for He), Rotary pump 30 L/min (60Hz)
• Column flow: Up to 4mL/min

GC/MS is an instrumental technique, comprising a Gas Chromatograph (GC) coupled to a Mass Spectrometer (MS), by which complex mixtures of chemicals may be separated, identified and quantified. This makes it ideal for the analysis of hundreds of relatively low molecular weight compounds found in environmental materials. For compound to be analysed by GC/MS it must be sufficiently volatile and thermally stable.

In addition, functionalised compounds may require chemical modification (derivatization), prior to analysis, to eliminate undesirable adsorption effects that would otherwise affect the quality of the data obtained. Samples (such as soils, sediments, tissues etc.) to be analysed need to be solvent extracted and the extract is subjected to various ‘wet chemical’ techniques prior to GC/MS analysis.

It assists to identify and quantify various molecules that are volatile in nature. This instrument can be used in studies relating to fatty acids and pesticides present in Biological, Environmental and Food matrices.

It is a widely used technique in the analysis of perfumes and essential oils.